Journal: Cell Death & Disease

Article Title: Rewiring melanoma cell fate: TRPM8 modulators trigger apoptosis and boost NK cell cytotoxicity

doi: 10.1038/s41419-026-08469-8

Figure Lengend Snippet: A Representative Western blot showing TRPM8 protein expression in the indicated cell lines. Tubulin was used as loading control. Viability of human melanocytes ( B ) and human dermal fibroblasts ( C ) untreated or treated with compounds 4 and 9 at the concentrations indicated in the legends on the right. Absorbance values from WST-1 assays at 24, 48, and 72 h are shown. Data are presented as mean ± SD of three independent experiments. n.s . indicates not significant. Representative Live/Dead assay images of human melanocytes ( D ) and human dermal fibroblasts ( E ) treated for 24 h with compounds 4 and 9 (1 or 10 μM). Viable cells are shown in green (acridine orange; total cells), while dead cells are shown in red (propidium iodide; dead cells). Overlay images are shown. Scale bar, 100 μm. Quantification of cell death is displayed to the right of each overlay image. The percentage of dead cells was calculated as: (red-stained dead cells/green-stained total cells) × 100.

Article Snippet: The Immortalized Human Dermal Fibroblasts ( P10858 -IM, Innoprot) were cultured in Fibroblast Medium-2 (FM-2; Innoprot) supplemented with 5% FBS (Gibco), 1% Fibroblast Growth Supplement-2 (FGS-2; Innoprot) and 1% penicillin-streptomycin (Gibco).

Techniques: Western Blot, Expressing, Control, Live Dead Assay, Staining

Journal: Cells

Article Title: The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling

doi: 10.3390/cells10061326

Figure Lengend Snippet: Activation of fibroblasts. huFib cells were treated with 1 µg/mL LPS, 500 ng/mL C0-Linker, or 500 ng/mL C0-C1f and/or 10 ng/mL TGFβ. ( A ) CXCL-1 ( B ) CCL-2 ( C ) MMP-9, and ( D ) ICAM-1 mRNA abundance was detected by qPCR after 24 h treatment. Data are displayed as individual data points and means; p -values above each condition refer to differences between means (horizontal lines) and 0 fold change (unstimulated controls) unless otherwise indicated. ( n = 4; n represents biological replicates).

Article Snippet: Immortalized human fibroblasts (huFib, Inscreenex, Braunschweig, Germany) were cultured at 37 °C with 5% CO 2 in huFib medium (Inscreenex) [ ].

Techniques: Activation Assay

Journal: Cells

Article Title: The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling

doi: 10.3390/cells10061326

Figure Lengend Snippet: Time-dependent response of fibroblasts. huFib cells were treated with 500 ng/mL C0-C1f, 500 ng/mL C0-Linker or 1 µg/mL LPS for different time periods, and ( A ) CXCL-1, ( B ) CCL-2, and ( C ) MMP-9 mRNA abundance was quantified by qPCR. Data are displayed as mean ± SD ( n = 4 biologically independent experiments). * above each condition refer to differences between means (data points) and 0 fold change (unstimulated controls). * p < 0.05 was considered statistically significant.

Article Snippet: Immortalized human fibroblasts (huFib, Inscreenex, Braunschweig, Germany) were cultured at 37 °C with 5% CO 2 in huFib medium (Inscreenex) [ ].

Techniques: